Sequences of mitochondrial small subunit ribosomal RNA (mt-SSU) were amplified from the two isolates by primers MS1 and MS2, and the sequences showed 99% similarity with Laetisaria fuciformis from the NCBI database (Accession No. The two isolates were successfully reisolated from all symptomatic tissues, completing Koch's postulates. Diseased plants in inoculated pots displayed symptoms similar to those observed in the field and no symptoms were detected on the control plants. Approximately 40% of leaves in inoculated pots were necrotic after 7 days, and this increased to 80% after 21 days. Two days after inoculation, the fungus was observed on the leaves. After inoculation, each pot was placed in a translucent plastic bag and placed into a greenhouse at 24 ± 2☌ with a 12-h photoperiod (1). Pots were inoculated by placing 2 g of infected grain within the center of the turf canopy or 2 g of sterilized, uninfested grains to serve as controls, with four replications of each treatment. Inoculum was prepared by culturing isolates separately on an autoclaved mixture of 100 g of rye grain and 20 ml of water for 3 weeks at 25☌. Seaspray) planted (0.5 mg seed/cm -2) in 10-cm pots. To confirm pathogenicity, isolates were inoculated on 6-week-old P. Two isolates from each golf course were collected by plating diseased leaf blades, stromata, or hyphal aggregates from the blighted leaves directly onto antibiotic (0.01% gentamicin sulfate) amended potato dextrose agar. The hyphae matted together, then formed threadlike or antlerlike stromata from the tips of blighted leaves. The green leaves of infected plants initially became water soaked, then tan to bleached, shriveled, and infested with pink or reddish, gelatinous, stranded hyphae. When morning dew was present or rainfall occurred, a pink layer of gelatinous fungal growth could be observed on leaves and sheaths. Salam on two golf courses in Haikou, Hainan Province, China. ![]() In April 2010, circular or irregular pink patches ranging from 5 to 50 cm in diameter were observed in the golf course fairway and rough established with cv. Because of its good texture and natural tolerance to various environmental stresses, seashore paspalum has been introduced to golf courses in coastal regions of southern China. Seashore paspalum (Paspalum vaginatum Swartz.) is a prostrate-growing, perennial, warm-season turfgrass native to tropical and coastal areas (2).
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